PhytophthoraV1, Main, Exploration, bibRecord, 000686

Protocol of Phytophthora capsici Transformation Using the CRISPR-Cas9 System.

Identifieur interne : 000686 ( Main/Exploration ); précédent : 000685; suivant : 000687

Protocol of Phytophthora capsici Transformation Using the CRISPR-Cas9 System.

Auteurs : Zhiwen Wang [République populaire de Chine] ; Brett M. Tyler [États-Unis] ; Xili Liu [République populaire de Chine]

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2018.

RBID : pubmed:30182240

Descripteurs français

KwdFr :
- ARN guide (MeSH), Mutation (MeSH), Ordre des gènes (MeSH), Phytophthora (génétique), Protoplastes (MeSH), Recombinaison homologue (MeSH), Systèmes CRISPR-Cas (MeSH), Transfection (MeSH), Transformation génétique (MeSH), Vecteurs génétiques (génétique), Édition de gène (MeSH).
MESH :
- génétique : Phytophthora, Vecteurs génétiques.
- ARN guide, Mutation, Ordre des gènes, Protoplastes, Recombinaison homologue, Systèmes CRISPR-Cas, Transfection, Transformation génétique, Édition de gène.

English descriptors

KwdEn :
- CRISPR-Cas Systems (MeSH), Gene Editing (MeSH), Gene Order (MeSH), Genetic Vectors (genetics), Homologous Recombination (MeSH), Mutation (MeSH), Phytophthora (genetics), Protoplasts (MeSH), RNA, Guide (MeSH), Transfection (MeSH), Transformation, Genetic (MeSH).
MESH :
- chemical : RNA, Guide.
- genetics : Genetic Vectors, Phytophthora.
- CRISPR-Cas Systems, Gene Editing, Gene Order, Homologous Recombination, Mutation, Protoplasts, Transfection, Transformation, Genetic.

Abstract

Phytophthora capsici is an important plant pathogen, which causes significant economic losses on multiple vegetable crops worldwide. It is an ideal model pathogen to study the role of important genes, plant-pathogen interactions, and fungicide resistance mechanisms etc. due to its wide range of hosts and genetic diversity. A more efficient gene editing tool is required to do these studies. Here, we describe a detailed experimental procedure using the CRISPR-Cas9 system to edit genes of interest in P. capsici, which has been proven to be an accurate and efficient gene editing method in P. capsici.

DOI: 10.1007/978-1-4939-8724-5_17
PubMed: 30182240

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Phytophthora capsici is an important plant pathogen, which causes significant economic losses on multiple vegetable crops worldwide. It is an ideal model pathogen to study the role of important genes, plant-pathogen interactions, and fungicide resistance mechanisms etc. due to its wide range of hosts and genetic diversity. A more efficient gene editing tool is required to do these studies. Here, we describe a detailed experimental procedure using the CRISPR-Cas9 system to edit genes of interest in P. capsici, which has been proven to be an accurate and efficient gene editing method in P. capsici.</div>
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Protocol of Phytophthora capsici Transformation Using the CRISPR-Cas9 System.

Protocol of Phytophthora capsici Transformation Using the CRISPR-Cas9 System.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki